BACKGROUND: The study of ascidians (Chordata. Tunicata) has made a considerable contribution to our understanding of the origin and evolution of basal chordates. To provide further information to support forward genetics in Ciona intestinalis. we used a combination of natural variation and neutral population genetics as an approach for the systematic identification of new mutations. https://www.roneverhart.com/Chicago-Bears-Adventure-Wagon-Portable-Utility-Wagon/